Field deployable quantitative polymerase chain reaction assays for harmful algae

Creator: Lincoln MacKenzie
Subject: Keywords: Sustainable Seas, Hydrogel qPCR assay, harmful algae, Alexandrium pacificum, Alexandrium minutum, Karena brevisulcata, Pseudochattonella verruculosa, Heterosigma akashiwo,
Description: Large subunit rDNA gene sequences used as primers for qPCR assays of shellfish poisoning and fish killing algae (Alexandrium pacificum, Alexandrium minutum, Karena brevisulcata, Pseudochattonella verruculosa, Heterosigma akashiwo). Standard curves and cross reactivity assays of cultured isolates in hydrogel format. Results of assays from laboratory and in situ field trials of the hydrogel assay on samples collected during surveys of A. pacificum blooms in Pelorus Sound. (Sustainable Seas project number IF2.2.2.5)
Publisher: Cawthron Institute
Type: Speadsheets with results of assays (Cq estimates) and plots of relationships between manual counts and genetic quantification. Screen shots of assay polymerisation and melt curves.
Format: .xlsx jpg
Source: Local Storage
Language: English
Modified: 2019-07-03T11:10:08